Objectives: - Share actual techniques used by ExAdEx-Innov company to monitor on a daily basis, the viability and physiology of human adipose tissue ex vivo culture
- What are the criteria at tissue level to evaluate adipose tissue viability
Introduction: ExAdEx-Innov is a biotech company specialized in applied research on human adipose tissue. We have developed the only technology that allows to keep real human adipose tissue viable and fully functional in culture during several weeks , so we have established differents procedures to evaluate AT viability at tissue level.
Materials / method: - Metabolic activity to evaluate viability of adipose tissue
- 3D confocal imaging to evaluate adipocyte size and volume, collagen composition and viability of vascular network
- In vitro assay of physiology of Adipose tissue
Results: - Scoring of adipose tissue viability based on metabolic activity, 3D structure, vascularization and functionnal assays
Conclusion: With our internal quality control, we can monitor in a reproductible and with human data at tissue level, the viability of an adipose tissue sample with less expensive and faster methods that are commonly used
Disclosures
Did you receive any funding to support your research for this TOPIC?
No
Were you provided with any honoraria, payment or other compensation for your work on this study?
No
Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No
Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No
This work is presented thanks to the support of: ExAdEx-Innov