IMCAS World Congress 2025
Program

Objectives: 1. To understand isolate and analyze the characteristics of ASC-EXO, and PSC-EXO
2. To evaluate the biological effects of ASC-EXO, and PSC-EXO on cell migration, proliferation, collagen synthesis, melanin inhibition, and anti-inflammatory activity in skin regenerative aesthetics.

Introduction: Adipose tissue-derived mesenchymal stem cell exosomes (ASC-EXO) and plant stem cell-derived exosome-like nanoparticles (PSC-EXO) have attracted significant interest for their therapeutic potential in regenerative medicine and aesthetics. This study explores the characteristics and biological functions of ASC-EXO and PSC-EXO, with an emphasis on their anti-inflammatory effects, enhanced cell proliferation, and promotion of collagen synthesis, highlighting their potential to improve skin health.

Materials / method: ASC-EXO and PSC-EXO isolated using the ExoSCRT tangential flow filtration (TFF) method. ASC-EXO were applied to in vitro assay models such as cellular uptake, cell proliferation and collagen synthesis in HDF, anti-inflammation assay in LPS-macrophage. In addition, the contents of ASC-EXO were analyzed through protein, miRNA, lipid profiling. PSC-EXO were applied to in vitro assay models such as cellular uptake, cell proliferation, and collagen synthesis in HDF in vitro model, anti-inflammation, and melanin synthesis. In addition, the contents of Plant-EXO were analyzed for miRNA and proteins.

Results: Characterization of ASC-EXO revealed a size range of 30-200 nanometers, with over 777 of the proteins identified by proteomic analysis. 132 of the miRNAs were identified by small RNA sequencing analysis. The top 20 miRNAs accounted for approximately 78% of the total, and the top five miRNAs let-7a-5p, let-7b-5p, miR-4301, and miR-23a-3p contributed to 46% of the counts. Mass spectrometry quantified 373 lipid species in ASC-EXO by lipidomics.
miRNA analysis revealed that Plant-EXO has about 30 kinds matched to human-specific miRNAs.

Conclusion: These findings suggest that both ASC-EXO and PSC-EXO have unique and beneficial properties for skin-related applications, highlighting their potential for development into therapeutic agents in regenerative medicine and aesthetic uses. The OMICS analysis of ASC-EXO and PSC-EXO provides a comprehensive perspective on enhancing skin quality. These data collectively show that Exosomes containing microRNA and proteins have multiple biological functions in skin-related assays such as fibroblast growth and melanin content in melanocytes.

Disclosures

Did you receive any funding to support your research for this TOPIC?
Yes
Please specify entities (individual, company, society): ExoCoBio Inc.

Were you provided with any honoraria, payment or other compensation for your work on this study?
Yes
Please specify entities (individual, company, society): ExoCoBio Inc.

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work is presented thanks to the support of: ExoCoBio Inc.

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work was not supported by any direct or non direct funding. It is under the author's own responsability

Objectives: This presentation aims to share best practices for integrating Exosome technology into aesthetic medicine. It will delve into the supporting clinical science underpinning the use of Exosome and provide valuable insights for practitioners seeking to optimize treatment outcomes with this innovative approach.

Introduction: Exosomes, extracellular vesicles containing a diverse array of bioactive molecules, have emerged as promising therapeutic agents in various fields, including aesthetics. Their potential lies in their ability to modulate cellular functions, promote tissue regeneration, and reduce inflammation. However, rigorous scientific evaluation is paramount for ensuring the safety and efficacy of exosome-based products. This necessitates careful consideration of source materials, meticulous manufacturing processes, and robust clinical data to support their use in aesthetic applications.

Materials / method: This presentation draws upon a comprehensive analysis of clinical experience with Exosome in various aesthetic applications. This includes a review of published literature, analysis of data from numerous case studies conducted in my own practice, and consideration of best practices observed across a global network of clinicians utilizing Exosome technology.

Results: Key findings include synergistic effects observed when combining Exosome with other aesthetic modalities, such as microneedling, laser therapy, and radiofrequency treatments. Furthermore, the efficacy of Exosome topical application in enhancing post-treatment healing, reducing inflammation, and accelerating tissue regeneration was demonstrated. Clinical data supports the safety and potential efficacy of Exosome in aesthetic applications.

Disclosures

Did you receive any funding to support your research for this TOPIC?
Yes
Please specify entities (individual, company, society): Benev

Were you provided with any honoraria, payment or other compensation for your work on this study?
Yes
Please specify entities (individual, company, society): Benev

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work is presented thanks to the support of: Benev

Objectives: Open-label split face clinical trial assessing the safety and efficacy of Adipose Stem Cell Derived Exosomes vs PRP post RF microneedling in the cosmetic improvement of facial photoaged skin

Introduction: Exosomes and P.RP are finding their roles in aesthetic medicine

Materials / method: 15 subjects FP I-IV between ages 44-68 years old. Subjects received 3 RF microneedling treatments to the full face spaced at 4-week intervals.
Post treatment, PRP applied to right side and exosomes to the left side of the face.
Followed for 6 months post final treatment. 4 biopsies were taken from the right & left infra-auricular area at: Baseline; After final treatment (Tx 3); 3-month follow-up and: 6-month follow-up.
Investigator and subject assessments were undertaken regarding overall skin appearance, wrinkling, dyschromia, erythema, and textural quality.

Results: Exosomes and PRP indicated comparable histological results. 
Exosomes showed slightly better results than PRP in Investigator visual assessments. 

Conclusion: Exosomes are less invasive as they do not require blood draw. 
Can result in equal or improved efficacy than PRP. 

PRP is harvested from the patient's own blood, reflecting their age, whereas exosomes are harvested from healthy donors aged 18 to 25 years, potentially offering more robust regenerative properties.

Disclosures

Did you receive any funding to support your research for this TOPIC?
Yes
Please specify entities (individual, company, society): Benev

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work was not supported by any direct or non direct funding. It is under the author's own responsability

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work was not supported by any direct or non direct funding. It is under the author's own responsability

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
Yes
Please specify entities (individual, company, society): Benev-ExecoBio

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work is presented thanks to the support of: Benev-ExecoBio