Objectives: * Provide an overview, update and analysis of the evidence for platelet rich plasma (PRP), exosomes and the secretome in hair restoration and skin rejuvenation through review of recent available data
* Identify and highlight controversies in the protocols for using these various tools in the aesthetic aspects of regenerative medicine
* Provide expert insights to guide future studies, and stimulate discourse and innovations benefitting patients.

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work was not supported by any direct or non direct funding. It is under the author's own responsability

Objectives: Attendees will learn how platelet concentration in platelet-rich plasma, (PRP), affects clinical efficacy and how to improve PRP quality using simple visual cues and centrifuge adjustments. They will gain practical tips for enhancing PRP concentration and clinical results, with optional insights into when and how hematology analysis might be beneficial.

Introduction: Although PRP is often praised as "liquid gold" for its regenerative benefits, research has shown that many PRP systems produce plasma with low platelet concentration, leading to suboptimal results. This presentation will uncover common shortcomings in PRP preparation and provide simple, practical strategies for increasing platelet concentration, using both visual cues and centrifuge techniques. While hematology analysis remains the gold standard for precise measurements, PRP quality can still be improved in most clinical settings, even without advanced equipment.

Materials / method: Drawing from over 10 years of experience and direct testing of thousands of PRP samples, this session highlights practical methods to assess and improve PRP quality. Visual cues, such as plasma opacity, and the presence or absence of platelet pellets, were studied for insights into PRP platelet concentration. Optimal PRP preparation techniques were developed using incremental changes in centrifuge settings (time and force) for optimal concentration. Simple visual cues and processing techniques were developed and refined to improve the quality of PRP.

Results: PRP systems vary widely in platelet concentration with a direct impact on clinical results. Although hematology analysis is the gold standard for testing and improving PRP concentration and quality, it is not available in many clinics. However there are still simple, practical techniques which can be used to elevate the quality of PRP, even without advanced testing equipment. These include visual inspection of PRP, simple centrifuge adjustments, and double-spin processing techniques. Each sample requires careful processing based on individual characteristics.

Conclusion: The quality and concentration of many common PRP systems may be known and improved using simple, practical methods such as visual cues, centrifuge adjustments, and double-spin processing, even in the absence of advanced hematology analysis. By applying these methods, clinicians can understand and elevate the quality of their PRP preparation, leading to improved clinical outcomes for their patients.

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work was not supported by any direct or non direct funding. It is under the author's own responsability

Objectives: PRP can be used for the treatment of chronic gynecological and reproductive diseases.
For example, at 37 years old, the ovary has about 450 scars from ovulation.
During aging and ovarian resistance, there are fewer granulose cells and fewer antral follicles.

Introduction: The cortex of the ovary is formed by the stroma, a connective-like tissue with many cells compared to the extracellular matrix, and it has few reticular and collagen fibers.
The medulla is the innermost area of the ovary, surrounded by the cortex.
Women begin the loss of collagen in their bodies earlier than men.

Materials / method: This is due to the decrease in hormone levels, which accelerates collagen degradation.
For menopausal women, collagen is almost absent in the ovarian cortex and vagina.
At this point, the injection of PRP in the ovarian is essential.

Results: Purpose: To investigate the impact of a 3-month course of intracortical injections of autologous platelet-rich plasma (PRP) plus exosomes platelets derivates upon ovarian reserve markers versus no intervention in women with low ovarian reserve before undergoing assisted reproductive technology (ART)

Conclusion: Conclusion PRP injections are effective and safe for improving markers of low ovarian reserve before ART.

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work was not supported by any direct or non direct funding. It is under the author's own responsability

Objectives: The appropriate techniques for each region of the face for rejuvenation and acne scars.

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work is presented thanks to the support of: RegenLab

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work was not supported by any direct or non direct funding. It is under the author's own responsability

Objectives: The study is to assess the lyophilized platelet injection's efficacy in skin rejuvenation through a split-face comparison

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No
Please specify entities (individual, company, society): Spirit Scientific, Taiwan

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work was not supported by any direct or non direct funding. It is under the author's own responsability

Objectives: For facial skin rejuvenation and anti-ageing, PRP alone has shown to improve texture, wrinkles, facial volume and skin color. Latest trends suggest the use of PRP in periorbital hyperpigmentation, lip rejuvenation, melasma, vitiligo and rosacea. Latest trends also recommend the use PRP in combination with skin boosters, CO2 lasers and microneedling for acne scarring and striae. PRP not only improves significantly patients’ facial skin indicators, but also quality of life, and satisfaction with their appearance.

Introduction: Platelet-rich plasma (PRP) is an autologous plasma product with concentration of platelets 3 to 7 times grater than baseline, obtained by centrifuging autologous blood before the procedure. PRP includes bioactive molecules such as chemokines, cytokines, and growth factors, able to stimulate cellular proliferation, differentiation, neoangiogenesis, activating fibroblasts and synthesizing collagen. These properties make PRP an attractive option for skin regeneration and rejuvenation. PRP also has a relatively low side effect profile.

Materials / method: For facial skin rejuvenation and anti-ageing, PRP alone has shown to improve texture, wrinkles, facial volume and skin color. Latest trends suggest the use of PRP in periorbital hyperpigmentation, lip rejuvenation, melasma, vitiligo and rosacea. Latest trends also recommend the use PRP in combination with skin boosters, CO2 lasers and microneedling for acne scarring and striae. PRP not only improves significantly patients’ facial skin indicators, but also quality of life, and satisfaction with their appearance.

Results: PRP combined with microneedling for atrophic acne scars shows better results than microneedling alone. Atrophic acne scars treated with PRP and fractional CO2 laser show shorter duration of erythema and edema, less post-inflammatory hyperpigmentation, and shorter recovery time. Intradermal PRP injections could also be a good alternative for treating melasma and vitiligo.

Conclusion: Questions have been posed on the efficacy in PRP use for striae distensea management. PRP not only improves significantly patients’ facial skin indicators, but also quality of life, and satisfaction with their appearance.

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work was not supported by any direct or non direct funding. It is under the author's own responsability

Objectives: The aim of this study is to quantitatively evaluate the PRP of vasculopathic patients on antiplatelet therapy and evaluate clinical implications.

Introduction: Despite numerous attempts to standardize the preparation methods of Platelet Rich Plasma (PRP), to date there is no homogeneity either in the techniques or in the production of dedicated kits and consequently in the quality of platelets concentrates. Added to this is the problem linked to patients with high cardiovascular risk on antiplatelet therapy. These are the ones who most frequently need PRP therapy but should they discontinue antiplatelets medication before blood harvesting for PRP preparation?

Materials / method: The study involves the enrollment of 10 vasculopathic patients, both male and female, mean age 58 yrs, before the start of antiplatelet therapy (acetylsalicylic acid 100 mg) and after 30 days from its start, and 10 healthy control patients not on therapy. A 10 cc sample of whole blood have been taken from all 20 patients
and collected in a tube with anticoagulant (sodium citrate 3.8%). The samples taken have been subjected to centrifugation by dedicated personnel and according to current legislation to obtain the plasma and to analyze platelets activity and growth factors.

Results: The number of platelets, their functionality and content of
growth factors have been evaluated, identifying any deficiencies. Furthermore a wound closure test has been performed to evaluate the functionality of PRP before and after 30 days of antiplatelets medication. The variation of growth factors between the 3 groups wasn't statistically significant.

Conclusion: These preliminary results could suggest that PRP can be effective also in patients under antiplatelets medication with acetylsalicylic acid 100 mg, leading to a wider possible utilization of PRP therapy. It is necessary increase the number of patients to reach a strong significativity of the results, considering the important clinical implications.

Disclosures

Did you receive any funding to support your research for this TOPIC?
Yes
Please specify entities (individual, company, society): Prize for best research project 2021/2022 ffrom Italian Society of Aesthetic Medicine SIME

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work is presented thanks to the support of: Italian Society of aesthetic medicine prize for best research project 2021/2011

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
Yes
Please specify entities (individual, company, society): Arthrex GmbH

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work is presented thanks to the support of: Arthrex GmbH